ABSTRACT
Objective To examine expression levels of autophagy gene pULK and PI3KC3 and to explore their correla?tion with non-small cell lung cancer (NSCLC). Methods A total of 77 samples of surgical resection from NSCLC speci? mens (including 31 cases of squamous cell carcinoma, 31 cases of adenocarcinoma and 15 cases of large cell undifferentiated carcinoma) and 21 samples of same normal lung tissue were randomly selected. Expressions of pULK and PI3KC3 in lung tissues were assessed by immunohistochemistry and Western blot. All dates were analyzed using SPSS13.0 statistical pack?age. Results Immunohistochemistry indicated that pULK and PI3KC3 localized into the cytoplasm. The expression levels of pULK and PI3KC3 are significantly lower in patient with NSCLC than those in peri-tumor tissue (35.1%vs 81.0%, 40.3%vs 76.2%respectively, P<0.01) . Immunohistochemistry and Western bolt analysis confirmed that pULK and PI3KC3 ex?pressions were significantly down-regulated (P<0.01) in patients with low grade cellular differentiation, metastasis of lymph node, or stageⅢandⅣ. And expression levels of pULK and PI3KC3 in NSCLC did not differ significantly with ages, gen?der, tumor size and pathological type. Correlation analysis showed that the expression of PI3KC3 was positively correlated with pULK. Conclusion pULK and PI3KC3 expressions were lower in NSCLC than those in normal lung tissue group. The expression levels of pULK and PI3KC3 in NSCLC were correlated with patient's clinical stage, differentiation grade, lymph node metastasis but were unrelated with age, gender, histological type and size.
ABSTRACT
Objective To investigate the effects of Ginsenoside Rg1 on the expression of nitric oxide synthase (NOS) in brain tissue after cerebral arterial thrombosis in adult rats. Methods Thirty rats were randomly divided into the sham-operative group, cerebral ischemia-reperfusion group, Ginsenoside Rg1-L group, Ginsenoside Rg1-M group, Ginsen-oside Rg1-H group and nimodipine group (n=5 for each group). The ischemia-reperfusion rat model was established by mid-dle cerebral artery occlusion. The neurological score after reperfusion was observed. The levels of nitric oxide (NO), neuronal NOS (nNOS) and inducible NOS (iNOS) were detected by nitrate reduction method and colorimetric method. The expressions of nNOS and iNOS after reperfusion were analyzed by immunohistochemistry and Western blot assay. Results (1) The neu-rological scores after cerebral ischemia were significantly lower in Rg1-L group, Rg1-M group and Rg1-H group than those of cerebral ischemia-reperfusion group(2.40±0.55,1.80±0.84, 1.60±0.89 vs 3.20±0.84,P<0.05). (2) Compared with those of model group, serum levels of NO and iNOS were reduced, and nNOS levels increased, in three groups of Rg1. (3) Compared with those of model group, the expression of nNOS was significantly increased,and iNOS expression was significantly re-duced, in three groups of Rg1. Conclusion The preventive effects of Ginsenosides Rg1 on cerebral ischemia-reperfusion injury may be associated with the activation of nNOS and the inhibition of iNOS.